The application of microfluidics to biotechnology is an exciting new area that has already begun to revolutionize how researchers study and manipulate macromolecules like DNA, proteins and cells in vitro and within living organisms. Now in a newly revised and expanded second edition, the Artech House bestseller, Microfluidics for Biotechnology brings you to the cutting edge of this burgeoning field. Among the numerous updates, the second edition features three entirely new chapters on: non-dimensional numbers in microfluidics; interface, capillarity and microdrops; and digital, two-phase and droplet microfluidics. Presenting an enlightening balance of numerical approaches, theory, and experimental examples, this book provides a detailed look at the mechanical behavior of the different types of micro/nano particles and macromolecules that are used in biotechnology. You gain a solid understanding of microfluidics theory and the mechanics of microflows and microdrops. The book examines the diffusion of species and nanoparticles, including continuous flow and discrete Monte-Carlo methods. This unique volume describes the transport and dispersion of biochemical species and particles. You learn how to model biochemical reactions, including DNA hybridization and enzymatic reactions. Moreover, the book helps you master the theory, applications, and modeling of magnetic beads behavior and provides an overview of self-assembly and magnetic composite. Other key topics include the electric manipulation of micro/nanoparticles and macromolecules and the experimental aspects of biological macromolecule manipulation.
Table Of Contents
Preface ; Acknowledgements ; Dimensionless Numbers in Microfluidics -Introduction. Microfluidic Scales. Buckingham 's Pi Theorem. Scaling Numbers and Characteristic Scales.; Microflows -Introduction. Single-Phase Microflows. Conclusion. ; Interfaces, Capillarity, and Microdrops -Introduction. Interfaces and Surface Tension. Laplace Law and Applications. Partial or Total Wetting. Contact Angle: Young 's Law. Capillary Force and Force on a Triple Line. Pinning and Canthotaxis. Microdrops. Conclusions. ; Digital, Two-Phase, and Droplet Microfluidics -Introduction. Digital Microfluidics. Multiphase Microflows. Droplet Microfluidics. Conclusions. ; Diffusion of Biochemical Species -Introduction. Brownian Motion. Macroscopic Approach: Concentration. Microscopic (Discrete) Approach. Conclusion. ; Transport of Biochemical Species and Cellular Microfluidics -Introduction. Advection-Diffusion Equation. Trajectory Calculation. Separation/Purification of Bioparticles. Cellular Microfluidics. Conclusion. ; Biochemical Reactions in Biochips -Introduction. From the Principle of Biorecognition to the Development of Biochips. Biochemical Reactions. Biochemical Reactions in Microsystems. Conclusion. ; Experimental Approaches to Microparticles-Based Assays - A Few Biological Targets. Microparticles as Biotechnological Tools. Experimental Methods of Characterization. Molecular Micromanipulation. ; Magnetic Particles in Biotechnology -Introduction. Characterization of Magnetic Beads. Magnetic Force. Deterministic Trajectory. Example of a Ferromagnetic Rod. Magnetic Repulsion. Magnetic Beads in EWOD Microsystems. Example of a Separation Column. Concentration Approach. Example of MFFF. Assembly of Magnetic BeadsMagnetic Beads Chains. Magnetic Fluids. Magnetic Micromembranes. Conclusion. ; Micromanipulations and Separations Using Electric Fields - Action of a DC Electric Field on a Particle: Electrophoresis. Dielectrophoresis. ; Conclusion. List of Symbols. About the Authors. Index;
Jean Berthier is a scientist at CEA/LETI and teaches at the University of Grenoble. He holds a masters degree in mathematics from the University of Grenoble and both an engineers and masters degree in fluid mechanics from the Institut National Polytechnique.
Pascal Silberzan is a senior scientist at the Physico-Chimie Curie Laboratory, a joint laboratory of the Centre National of the Recherche Scientifique and the Institut Curie in Paris. He received his Ph.D. from the College of France and the University Paris.