Microfabrication technology has quietly changed the world around us. Hiding under the shiny coat of our cars, cellular phones, and music players, the silicon microchip markedly changed our way of life. Features a thousand times smaller than a single millimeter enable an unparallel control over electrical signals resulting in nearly magical computational, communication, and memory powers. At the dawn of this century, a similar revolution is changing the study of biology and the practice of medicine. Microscale patterns, three-dimensional features, and the physics of small places offer a unique ability to control the microenvironment providing innovative tools for the study, diagnosis and treatment of disease. Offering a practical look into the field, this volume presents the science behind microscale device design and the engineering of its fabrication. Supported with dozens of full-color illustrations, this book offers you clear, step-by-step methods for: Cell capture from whole blood; High-throughput study of transcriptional dynamics in living cells; Temporal control of cell-cell interaction; Nanoscale measurements of cellular forces; Immobilizing living c. elegans; Optical and electrical on-chip cell sorting; Human-on-chip modeling of drug metabolism.
Preface ; Immunoaffinity Capture of Cells from Whole Blood -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. ; Dynamic Gene-Expression Analysis in a Microfluidic Living Cell Array (mLCA) -Introduction. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion. Application Notes. Summary Points. ; Micromechanical Control of Cell-Cell Interactions -Introduction. Experimental Design. Materials. Methods. Discussion. Summary Points. ; Mechanotransduction and the Study of Cellular Forces -Introduction. Materials. Methods. Discussion. Summary Points. ; A Microfluidic Tool for Immobilizing C. elegans -Introduction. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. ; Osmolality Control for Microfluidic Embryo Cell Culture Using Hybrid Polydimethylsiloxane (PDMS)-Parylene Membranes -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. ; Image-Based Cell Sorting Using Microscale Electrical and Optical Actuation -Introduction. Materials. Experimental Design. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Summary Points. ; Pharmacokinetic-Pharmacodynamic Models on a Chip -Introduction. Pharmacokinetic-Pharmacodynamic Modeling. Micro Cell Culture Analog (_CCA). Application Notes. Summary Points. ; Lab-on-a-Chip Impedance Detection of Microbial and Cellular Activity -Introduction. Lab-on-a-Chip for Monitoring Microbial Metabolic Activity. Lab-on-a-Chip for Impedance Detection of Cell Concentration Based on Ion Release from Cells. Conclusion. Summary Points. ; Controlling the Cellular Microenvironment -Introduction. Microenvironmental Control of Cell-Cell Interactions. Interactive Use of Substrate Topography and Electrical Stimulation for the Control of Cell Alignment. ; Subtractive Methods for Forming Microfluidic Gels of Extracellular Matrix Proteins -Introduction. Materials. Methods. Anticipated Results. Application Notes. Discussion and Commentary. Summary Points. ; About the Editors. List of Contributors. Index ;
-
Sangeeta Bhatia
Sangeeta Bhatia is a Howard Hughes Medical Institute Investigator and a professor in the Department of Health Sciences and Technology, as well as the Department of Electrical Engineering and Computer Science, at MIT. She is associate member of the Broad Institute and Brigham & Women 's Hospital and the director of the Laboratory for Multiscale Regenerative Technologies. Dr. Bhatia holds an M.D. from Harvard Medical School and a Ph.D. in medical engineering from the Harvard-MIT Division of Health Sciences and Technology.
-
Yaakov Nahmias
Yaakov Nahmias is an instructor in Surgery and Bioengineering at the BioMEMs Resource Center at Massachusetts General Hospital, Harvard Medical School. He is a senior lecturer at the School of Computer Science and Engineering at the Hebrew University of Jerusalem, and an associate member of the Center for Bioengineering in the Service of Humanity. Dr. Nahmias holds a B.Sc. in chemical engineering from the Technion-Israel Institute of Technology and a Ph.D. in biomedical engineering from the University of Minnesota.