Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This first-of-its-kind volume addresses the important challenge of organ preservation and reengineering. The book presents cutting-edge techniques for damaged livers and hearts via normothermic perfusion, hypothermic machine perfusion for the liver, kidney and pancreas, and imaging techniques to assess the viability of injured kidneys. Professionals and researchers also find methods to decellularize whole organs to create scaffolds for tissue engineering. Moreover, the book presents an approach to enhancing hepatocyte recovery from marginal livers.
Normothermic Machine Perfusion of the Liver -Introduction. Experimental Design and Methods. Data AcquisitionAnticipated Results and Interpretation. Discussion and Commentary.; Heart Preservation: Conventional Versus Novel Techniques -Introduction. Materials and Methods. Discussion and Commentary. Summary Points.; Hypothermic Machine Perfusion of Kidneys -Introduction. Experimental Methods. Discussion and Commentary. Application Notes. Summary Points.; Hypothermic Machine Perfusion of Livers -Introduction. Experimental Design. Materials. Methods. Data Analysis. Anticipated Results. Discussions and Commentary. Application Notes. Summary Points.; Hypothermic Perfusion of Pancreas: Emphasis on Preservation Prior to Islet Isolation -Introduction. Experimental Methods and Materials. Data Acquisition and Anticipated Results. Discussion and Commentary. Troubleshooting. Application Notes. Summary Points.; Methods of Cardiac Oxygen Persufflation -Introduction. Materials. Methods. Data Acquisition and Results. Discussion and Commentary. Summary Points.; Vascular Oxygen Persufflation for Preservation and Reconditioning of Marginal Liver Grafts -Introduction. Materials. Methods. Discussion and Commentary. Application Notes. Summary Points.; Use of Optical Imaging and Spectroscopy in Assessment of Organ Perfusion -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Summary Points.; Preparation of a Transplantable Liver Graft by the Recellularization of a Decellularized Whole Organ -Introduction. Methods. Anticipated Results. Discussion and Commentary.; Engineering Lung Tissue -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; Detergent-Enzymatic Method for Bioengineering Human Airways -Introduction. Experimental Design. Reagents. Methods. Anticipated Results. Discussion and Commentary. Clinical Notes. Summary Points.; Ex Vivo Perfusion of Rat Liver Grafts for Hepatocyte Retrieval -Introduction. Experimental Design. Methods. Data Acquisition. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; Technique of Clinical Vascular Isolation and Perfusion of the Liver -Introduction. Typical Patient Experience. Materials. Methods. Data Acquisition, Interpretation, and Patient Management During Perfusion. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; About the Editors. Index.;
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Charles Y. Lee
Charles Y. Lee is an associate professor in the Department of Medical Engineering at the University of North Carolina, Charlotte. Dr. Lee earned his Ph.D. in mechanical engineering from the University of California, Berkeley.
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Korkut Uygun
Korkut Uygun is the leader of the Organ Engineering Group at Massachusetts General Hospital, where he was instrumental in developing a technique that promises to allow growth of transplantable replacement livers. Dr. Uygun received his Ph.D. in chemical engineering from Wayne State University.